How large should.....
). As long as you get no problems, and you can work through the map ok, everything should be fine.
If you chambers play just fine as separate map files, it's the better way to go.
lets say you forgot one simple fizzler and the player (for no real reason) finds a way to get all the way back to the beggining of your map and then get stuck or some such junk. just cuz the map was too big...
now i'm not speaking against big maps. some of them i really like just saying that the more you put, the more chance we will have to break your map eg
taco wrote:
...... If you chambers play just fine as separate map files, it's the better way to go.
i agree. but likewise if you are having no problem with your LARGE map.. then bring it on 
Its up to you how big you make your map files, its a trade off between how often you want to see the loading screen or how long you want the loading screen to take.
One large level per load seems fine and for smaller levels two per load seems fine, just like valve did. what i wouldnt do however is have half of a chamber in one bsp and the other half of the same chamber inanother bsp, that would be bad.